Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Using immunocytochemical methods at the electron microscope level, immunoreactivity for both melanin-concentrating hormone (MCH) and alpha-melanocyte-stimulating hormone (alpha-MSH) has been demonstrated in the carp neurohypophysis. A double-labelling technique, using colloidal gold probes of different sizes showed that immunoreactivity to both molecules coexists within the same neurosecretory granules in some neurones, while in other neurones the granules exhibit only MCH-like immunoreactivity. These observations suggest that the two immunoreactivities are attributable to separate molecules; if they are derived from the same precursor molecule, then this must be cleaved differently in the two sets of neurones. The absence of adrenocorticotropic hormone (ACTH)-like immunostaining in any neurosecretory granule might suggest the alpha-MSH-like molecule is not derived from the conventional pro-opiomelanocortin precursor.

Original publication

DOI

10.1016/0304-3940(87)90466-6

Type

Journal article

Journal

Neurosci Lett

Publication Date

05/10/1987

Volume

80

Pages

268 - 274

Keywords

Animals, Carps, Cytoplasmic Granules, Eels, Hypothalamic Hormones, Immunohistochemistry, Melanins, Melanocyte-Stimulating Hormones, Microscopy, Electron, Neurons, Pituitary Gland, Pituitary Hormones, Trout