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Dengue is the most prevalent arboviral disease of humans. The host and virus variables associated with dengue virus (DENV) transmission from symptomatic dengue cases (n = 208) to Aedes aegypti mosquitoes during 407 independent exposure events was defined. The 50% mosquito infectious dose for each of DENV-1-4 ranged from 6.29 to 7.52 log10 RNA copies/mL of plasma. Increasing day of illness, declining viremia, and rising antibody titers were independently associated with reduced risk of DENV transmission. High early DENV plasma viremia levels in patients were a marker of the duration of human infectiousness, and blood meals containing high concentrations of DENV were positively associated with the prevalence of infectious mosquitoes 14 d after blood feeding. Ambulatory dengue cases had lower viremia levels compared with hospitalized dengue cases but nonetheless at levels predicted to be infectious to mosquitoes. These data define serotype-specific viremia levels that vaccines or drugs must inhibit to prevent DENV transmission.

Original publication

DOI

10.1073/pnas.1303395110

Type

Journal article

Journal

Proc Natl Acad Sci U S A

Publication Date

28/05/2013

Volume

110

Pages

9072 - 9077

Keywords

entomology, infectious disease, virology, Aedes, Animals, Base Sequence, Cohort Studies, Dengue, Dengue Virus, Enzyme-Linked Immunosorbent Assay, Host-Pathogen Interactions, Humans, Insect Vectors, Likelihood Functions, Models, Genetic, Molecular Sequence Data, Phylogeny, Prevalence, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Serotyping, Vietnam, Viremia