In addition to our core equipment, listed below, the Peter Medawar Building runs a Shared Cytometry Resource Facility, run by David Malone. Within the facility we have a number of cytometers:

BD LSRII: 15 colour cytometer:

LSRII

LASER       FLUOROCHROME

PMT

BP FILTER

LP MIRROR

 

FSC

diode

 

none

488 nm

 

H

none

none

 

SSC

G

488/10

none

 

FITC

F

530/30

505

 

PE

E

575/26

550

 

PE-Texas Red

D

610/20

600

 

PE-Cy5/PerCP

C

660/20

655

 

PerCP-Cy5.5

B

695/40

685

 

PE-Cy7

A

780/60

735

633 nm

APC-Cy7/H7

C

780/60

755

 

AlexaFluor 700

B

730/45

710

 

APC

A

660/20

none

405 nm

 

H

none

none

 

 

G

none

none

 

Pacific Blue

F

450/50

none

 

Amycan

E

525/50

475

 

Qdot 565

D

560/20

545

 

QDot585

C

585/15

575

 

Qdot 605

B

605/12

599

 

Qdot 655

A

655/8

630

 

 

MACSQuant
MACSQuant

Miltenyi MACSQuant Analyzer 10:

8 colour cytometer with high throughput MiniSampler, and absolute cell counts .

 MACSQuant_details


Beckman Coulter MoFlo XDP:

A Jet-in-air, 4 way sorter, within a derogated Cat3 room - housed within a Class II safety cabinet. Setup:

XDP

488nm laser

- FITC
- PerCP/PerCP-Cy5.5
 561nm laser
- PE
- PE-TexasRed
- PE-Cy5
- PE-Cy7

 405nm laser

- Pacific Blue
- Pacific Orange
- QDot605

 633nm laser

- APC
- Alexar700
- APC-Cy7
 

 ZellScanner One:

High content cytometry for explorative analysis of cytometric and histological samples to understand structures, functions, and mechanisms on a cellular level.

Zellscanner

 

For further information about using this equipment, please contact David Malone (david.malone@paediatrics.ox.ac.uk)