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The effects of the immunocompromised state after liver transplantation on the frequency of cytomegalovirus-specific cytotoxic T lymphocytes (CTL) were investigated in 93 patients by using HLA class I tetrameric complexes corresponding to HLA-A*0201, HLA-B*0702, HLA-B*0801, and HLA-B*3501 refolded with peptides from the ppUL83 matrix protein. ppUL83 CTL frequencies were suppressed during the first 6 months after transplantation. Patients with >1 HLA-restricted response detected had high correlation among ppUL83 CD8(+) CTL frequencies restricted by different HLA haplotypes (Spearman's rho=.67; P<.0001). There was an inverse correlation among levels of the calcineurin inhibitor, tacrolimus, and ppUL83 CD8(+) CTL frequencies (r=-.31; P=.005), which is consistent with the presence of a large proportion (70%) of activated (CD38(+)) ppUL83 CD8(+) CTL within the population of HLA class I tetramer-positive cells.

Original publication

DOI

10.1086/319260

Type

Conference paper

Publication Date

15/03/2001

Volume

183

Pages

835 - 843

Keywords

Adolescent, Adult, Aged, Antigens, Viral, Cells, Cultured, Cytomegalovirus, Cytomegalovirus Infections, Histocompatibility Antigens Class I, Humans, Immunocompromised Host, Immunosuppressive Agents, Interferon-gamma, Liver Transplantation, Longitudinal Studies, Lymphocyte Count, Middle Aged, Phosphoproteins, T-Lymphocytes, Cytotoxic, Tacrolimus, Time Factors, Viral Load, Viral Matrix Proteins