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Serum antibodies from 1071 people in two Kenyan villages were assayed using eight different recombinant Apical Membrane Antigen 1 (AMA1) protein constructs to investigate their role in naturally acquired immunity. In both communities, antibodies against the full-length ectodomain (both FVO and 3D7 allele constructs) prior to a malaria transmission season were significantly associated with protection from malaria in the following 6 months, even after adjusting for age and antibody reactivity to whole parasite (schizont) extract. However, these protective associations of antibodies were only seen among subjects that were parasite slide positive at the time of pre-season serum sampling. Competition ELISAs with the FVO and 3D7 allele constructs showed that antibodies can recognise either conserved or allele-specific epitopes in AMA1. Results encourage the development of an AMA1 vaccine based on the full-length ectodomain, and indicate that the function of human antibodies to allele-specific and conserved epitopes in AMA1 should be studied further.

Original publication

DOI

10.1016/j.vaccine.2004.05.031

Type

Journal article

Journal

Vaccine

Publication Date

16/12/2004

Volume

23

Pages

718 - 728

Keywords

Adolescent, Adult, Aged, Animals, Antibodies, Protozoan, Antigens, Protozoan, Child, Child, Preschool, Cohort Studies, Enzyme-Linked Immunosorbent Assay, Epitopes, Humans, Infant, Infant, Newborn, Kenya, Malaria Vaccines, Malaria, Falciparum, Membrane Proteins, Middle Aged, Plasmodium falciparum, Protozoan Proteins, Seroepidemiologic Studies