Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

CD161(++) CD8(+) T cells represent a novel subset that is dominated in adult peripheral blood by mucosal-associated invariant T (MAIT) cells, as defined by the expression of a variable-α chain 7.2 (Vα7.2)-Jα33 TCR, and IL-18Rα. Stimulation with IL-18+IL-12 is known to induce IFN-γ by both NK cells and, to a more limited extent, T cells. Here, we show the CD161(++) CD8(+) T-cell population is the primary T-cell population triggered by this mechanism. Both CD161(++) Vα7.2(+) and CD161(++) Vα7.2(-) T-cell subsets responded to IL-12+IL-18 stimulation, demonstrating this response was not restricted to the MAIT cells, but to the CD161(++) phenotype. Bacteria and TLR agonists also indirectly triggered IFN-γ expression via IL-12 and IL-18. These data show that CD161(++) T cells are the predominant T-cell population that responds directly to IL-12+IL-18 stimulation. Furthermore, our findings broaden the potential role of MAIT cells beyond bacterial responsiveness to potentially include viral infections and other inflammatory stimuli.

Original publication

DOI

10.1002/eji.201343509

Type

Journal article

Journal

Eur J Immunol

Publication Date

01/2014

Volume

44

Pages

195 - 203

Keywords

CD161++ T cells, IL-12, IL-18, MAIT cells, T cells, CD8 Antigens, Cell Line, Cell Separation, Flow Cytometry, Humans, Interferon-gamma, Interleukin-12, Interleukin-18, Lymphocyte Activation, Mucous Membrane, NK Cell Lectin-Like Receptor Subfamily B, Natural Killer T-Cells, Receptors, Antigen, T-Cell, alpha-beta, Receptors, Interleukin-18, T-Lymphocyte Subsets