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Hepatitis C virus (HCV) genotype 6a is found in a restricted part of South East Asia, including Hong Kong, Macau and Vietnam. We determined the full length coding sequence of a type 6a isolate (EUHK2) obtained from a Hong Kong blood donor. The sequence of EUHK2 contained a single open reading frame coding for a polyprotein of 3018 amino acids, within the range of 3008 to 3037 for other HCV genotypes. The full length sequence of EUHK2 showed 30.3%-32.9% nucleotide (24.3%-29.4% amino acid) sequence divergence from genotypes 1-4, but only 27.7% (20.7% amino acid) divergence from JK046 ("type 11a"). These similarity values were intermediate between those of other HCV genotypes (minimum 28.4%) and between subtypes (maximum 25%). The close evolutionary relationship of EUHK2 with JK046 was further indicated by their grouping together by phylogenetic analysis.

Original publication

DOI

10.1006/bbrc.1997.6627

Type

Journal article

Journal

Biochem Biophys Res Commun

Publication Date

19/05/1997

Volume

234

Pages

393 - 396

Keywords

Cloning, Molecular, DNA, Viral, Evolution, Molecular, Genotype, Hepacivirus, Hong Kong, Humans, Molecular Sequence Data, Open Reading Frames, Phylogeny, Polymerase Chain Reaction, Sequence Homology, Amino Acid, Viral Proteins