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Crystals have been obtained of an antibody Fab fragment grown in the presence of a single domain from streptococcal protein G and a ten amino-acid peptide corresponding to the P1.7 serosubtype antigen from the human pathogen Neisseria meningitidis. Crystal trials using the Fab fragment and peptide antigen alone were unsuccessful, but the inclusion of a protein G domain provided an additional variable that generated suitable crystals. Crystals are in space group P21 with unit-cell parameters a = 43.60, b = 63.42, c = 89.63 A, beta = 98.58 degrees and a data set has been collected to 2.9 A resolution using synchrotron radiation. The inclusion of protein G is likely to be of general utility for the crystallization of Fab-antigen complexes.

Original publication

DOI

10.1107/S0907444998010269

Type

Journal article

Journal

Acta Crystallogr D Biol Crystallogr

Publication Date

01/1999

Volume

55

Pages

314 - 316

Keywords

Amino Acid Sequence, Animals, Antigen-Antibody Complex, Antigens, Bacterial, Bacterial Proteins, Crystallization, Crystallography, X-Ray, Epitopes, Humans, Immunoglobulin Fab Fragments, Mice, Molecular Sequence Data, Neisseria meningitidis, Streptococcus